Anti-Vascular Endothelial Growth Factor Receptor 1 antibody produced in goat

产品说明

一般描述

Vascular endothelial growth factors receptors (VEGF Rs) are receptor tyrosine kinases that bind to VEGF that plays important role in embryonic vasculogenesis, angiogenesis and homeostasis. The biological effects of VEGF are mediated by VEGF R1 and VEGF R2 that have the ability to heterodimerize. The binding of VEGF to VEGF R1 results in activation of tyrosine amino acids of the cytoplasmic tail of the receptor. This interaction results in the activation of MAPK, PI3K, PKC, FAK and Src kinase pathways. The biological roles of VEGF R1 include in epithelial cell growth, proliferation, survival, migration and vascular permeability and angiogenesis.
Anti-Vascular Endothelial Growth Factor Receptor-1 recognizes mouse VEGF R1. Based on ELISA, the antibody shows <7% cross reactivity with recombinant human VEGF R1, 4% cross-reactivity with recombinant mouse VEGF164, 1% cross-reactivity with recombinant rat VEGF164, and 0.2% cross-reactivity with recombinant mouse VEGF R2 and VEGF R3.

特异性

The antibody will neutralize the bioactivity of recombinant mouse VEGF R1 and can be used in the blockade of receptor-ligand interaction.

免疫原

purified recombinant mouse VEGF R1 extracellular domain expressed in NSO cells.

应用

Anti-Vascular Endothelial Growth Factor Receptor 1 antibody may be used in immunoblotting at a working concentration of 0.1-0.2 μg/ml. For flow cytometry the recommended concentration is 3-10 μg/ml. For ELISA, a working concentration of 0.8 μg/ml may be used. The antibody is suitable for neutralization reactions.

外形

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalsoe.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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