产品说明
一般描述
Recombinant, human p34Cdk1/cyclin B expressed in S. frugiperda insect cells. This kinase is a major regulator of the eukaryotic cell cycle. As a monomer, p34cdc2 is inactive and requires association with cyclin B to form an active complex.
Recombinant, human p34Cdk1/cyclin B expressed in S. frugiperda insect cells. This kinase is a major regulator of the eukaryotic cell cycle. As a monomer, Cdk1 is inactive and requires association with cyclin B and phosphorylation of Thr161 to form an active complex. MW: 34000(p34Cdk1) and 55000(cyclinB).
包装
200 u in Plastic ampoule
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of enzyme that catalyzes the transfer of 1 pmol of phosphate to histone H1 (10 µM) in 1 min at 30°C, pH 7.5.
外形
In 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM EDTA, 50% glycerol, 0.01% BRIJ® 35 Detergent, pH 7.5.
重悬
Following initial thaw, aliquot and freeze (-70°C).
其他说明
Kobayashi, H., et al. 1994. J. Biol. Chem. 269, 29153.
Atherton-Fessler, S., et al. 1993. Mol. Cell. Biol. 13, 1675.
法律信息
Brij is a registered trademark of Croda International PLC
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
| 形式 | liquid |
| 质量水平 | 100 |
| specific activity | ≥20,000 U/mL |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
| 异质活性 | Protease, phosphatase, none detected |
| 运输 | wet ice |
| 储存温度 | −70℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
