产品说明
一般描述
Recombinant, human caspase-2 expressed in E. coli as a single polypeptide chain. Subsequently undergoes spontaneous auto-processing to yield the native active enzyme. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. Cleavage sites are located at Asp316, Asp330, and possibly Asp152. MW: 12000 and 19000.
包装
100 u in Plastic ampoule
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of enzyme that will cleave 1.0 nmol of Ac-VDVAD-pNA (Cat. No. 218820) per h at 37°C, pH 7.2.
重悬
Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.
其他说明
Talanian, R.V., et al. 1997. J. Biol. Chem.272, 9677.
Wang, L., et al. 1994. Cell78, 739.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
| 质量水平 | 100 |
| 测定 | ≥90% (SDS-PAGE) |
| 形式 | lyophilized solid |
| specific activity | ≥1500 units/mg protein |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze |
| 溶解性 | PBS: 0.1-1 units/μL |
| 运输 | wet ice |
| 储存温度 | −70℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
