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Caspase-10, Human, Recombinant, E. coli

品牌
Sigma-Aldrich
货号
218785
规格纯度
Caspase-10, Human, Recombinant is expressed in E. coli as a single polypeptide. Fused at the N- and C- termini to a His·Tag sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.
参考价格
7750.5 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

M.W. 11,000 and 18,000 (heterodimer).
Recombinant, human caspase-10 fused at the N- and C- termini to a His•Tag sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.

包装

3000 u in Plastic ampoule

警告

Toxicity: Standard Handling (A)

单位定义

One unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-IETD-pNA (Cat. No. 368057) per min at 25°C, pH 7.4.

外形

In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.1% CHAPS, 0.09% APO-10, pH 7.4.

重悬

Following initial thaw, aliquot and freeze (-70°C).

其他说明

Kang, J.J., et al. 1999. J. Biol. Chem. 274, 3189.
Ng, P.W., et al. 1999. J. Biol. Chem. 274, 10301.
Vincenz, C., and Dixit, V.M. 1997. J. Biol. Chem. 272, 6578.
Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

质量水平100
测定≥95% (SDS-PAGE)
形式liquid
specific activity≥150,000 units/mg protein
≥50,000 units/mL
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
avoid repeated freeze/thaw cycles
运输wet ice
储存温度−70℃

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2

Sigma-Aldrich

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