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Amicon® Ultra-4 Centrifugal Filter Unit

Amicon® Ultra-4 离心过滤装置

品牌
Millipore
货号
UFC8003
规格纯度
Ultracel-3 regenerated cellulose membrane, 4 mL sample volume
参考价格
726.59 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Amicon® Ultra 4 mL centrifugal devices with Ultracel® 3,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.

应用

  • Ultrafiltration with 3,000 Dalton molecular weight cutoff Ultracel® membrane.
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
  • Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
  • Concentration of dilute or pre-purified proteins from column eluents.
  • Desalting, buffer exchange and protein dialysis.
  • Size exclusion and protein fractionation.

特点和优势

  • Ultracel® regenerated cellulose membrane with 3 kDa NMWCO delivers retentate recovery of greater than 90%.
  • Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).
  • Heat-sealed membrane minimizes downstream extractables.
  • 100% integrity tested for reliable performance.
  • Convenient sample monitoring with translucent housing and volume gradations.
  • Direct pipette sample access eliminates processing step to recover concentrate.
  • High concentration factors of 80–100X.

外形

色码:透明,带有粉色盖

法律信息

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

eCl@ss36100101
NACRESNB.24

产品性质

质量水平400
物料polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
无菌性non-sterile
产品线Amicon®
manufacturer/tradenameAmicon® Ultra
technique(s)protein extraction: suitable
protein purification: suitable
长度124 mm (4.88 in.)
直径17.3 mm
过滤面积3 cm2
孔径3 kDa NMWCO
试验参数sample volume: 4 mL
运输ambient

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