产品说明
一般描述
Filter Type: 超滤
Amicon® Ultra 4 mL centrifugal devices with Ultracel® 10,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Cytochrome C (12.4 kDa) at 0.25 mg/ml initial concentration is retained at >95% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
应用
- Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
特点和优势
- Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%.
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).
- Heat-sealed membrane minimizes downstream extractables.
- 100% integrity tested for reliable performance.
- Convenient sample monitoring with translucent housing and volume gradations.
- Direct pipette sample access eliminates processing step to recover concentrate.
- High concentration factors of 80–100X.
外形
Color Code: 透明，带有粉色盖
法律信息
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| eCl@ss | 36100101 |
| NACRES | NB.24 |
产品性质
| 质量水平 | 500 |
| 物料 | polyethylene cap polyethylene liner polypropylene tube regenerated cellulose membrane regenerated cellulose support styrene-butadiene housing |
| 无菌性 | non-sterile |
| 产品线 | Amicon® |
| manufacturer/tradename | Amicon® Ultra |
| technique(s) | protein extraction: suitable protein purification: suitable |
| 长度 | 124 mm (4.88 in.) |
| 直径 | 17.3 mm |
| 过滤面积 | 3 cm2 |
| 孔径 | 10 kDa NMWCO |
| 试验参数 | sample volume: 4 mL |
| 运输 | ambient |
安全信息
| 储存分类代码 | 10-13 - German Storage Class 10 to 13 |
