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pPolh-FLAGTM-1 Transfer Vector

品牌
Sigma-Aldrich
货号
T6824
包装型号
规格纯度
参考价格
6210.73 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

The pPolh-FLAG-1 vector contains the strong viral polyhedrin (polh) promoter for high-level expression of target genes during the very late phase of infection. The vector also contains a high copy bacterial origin of replication and an ampicillin resistance gene (ampr) for easy propagation in Escherichia coli host cells. Following co-transfection with linear baculovirus DNA into insect cells, allelic replacement between homologous viral sequences (AcNPV ORF 603 and ORF 1629) in the vector and the baculovirus DNA transfers the FLAG-target gene fusion sequence into the viral genome.

应用

pPolh-FLAG-1 is a 5575 bp baculovirus transfer vector used for producing FLAGÒ fusion proteins in insect cells. The vector is designed to be compatible with most baculoviral DNA systems that require the essential gene ORF1629 for complementation of lethal deletions and the recovery of viable recombinant virus. It is also a transfer vector for generation of recombinant Baculovirus and expression of N-terminal FLAG® fusions in insect cells.

法律信息

FLAG is a registered trademark of sigma-aldrich Co. LLC
pPolh-FLAG is a trademark of sigma-aldrich Co. LLC

基本信息

NACRESNA.32

产品性质

web metadata keyword.defaultbaculovirus recombinant vector, N-terminal FLAG fusions, insect recombinant protein expression
质量水平200
运输dry ice
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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