产品说明
应用
TrkB/Fc Chimera human can be used in synaptoneurosome preparation and western blot analysis. Intracerebroventricular administeration of TrkB/Fc chimera human blocks TrkB ligand signaling and was used to study the role of BDNF in ATN response augmentation.
生化/生理作用
Member of the Trk family tyrosine kinase receptors. Binds specifically to mediate neurotrophins BDNF and NT-4/5.
TrkB/Fc Chimera human consist of extracellular domain of human TrkB1 fused to histidine-tagged Fc region of human IgG1 by using a polypeptide linker. TrkB belongs to tyrosine kinase receptors family and facilitates the hippocampal development as well as regulating the synaptic plasticity that underlies long-term potentiation and learning. Brain derived neurotrophic factor (BDNF) and Neurotrophin 4 or 5 are preferred ligands for TrkB. Nevertheless it can also bind to NTF3/neurotrophin-3 that is less efficient in activating the receptor but regulates neuron survival through NTRK2.
其他说明
Extracellular domain of human TrkB fused to the C-terminal histidine-tagged Fc region of human IgG1.
外形
Lyophilized from 0.2 μm filtered solution in phosphate buffered saline.
重悬
Reconstitute to 100 μg/mL in phosphate buffered saline.
分析说明
Inhibits BDNF-induced proliferation of TrkB transfected cells, BaF-TrkB-BD.
基本信息
| MDL编号 | MFCD03458837 |
| NACRES | NA.32 |
产品性质
| 质量水平 | 200 |
| 生物来源 | human |
| 重组 | expressed in NSO cells |
| 测定 | >90% (SDS-PAGE) |
| 形式 | lyophilized powder |
| 效能 | 0.1-0.4 μg/mL ED50 |
| 分子量 | 120-130 kDa by SDS-PAGE (reducing) calculated mol wt 71 kDa |
| 包装 | pkg of 100 μg |
| 储存条件 | avoid repeated freeze/thaw cycles |
| technique(s) | western blot: suitable |
| 杂质 | endotoxin, tested |
| UniProt登记号 | Q16620 |
| 储存温度 | −20℃ |
| Gene Information | human ... NTRK2(4915) |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
