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Trypsin Agarose

品牌
Sigma-Aldrich
货号
T1763
规格纯度
buffered aqueous suspension, from bovine pancreas trypsin
参考价格
1257.05 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Trypsin Agarose is an insoluble enzyme product. It is produced by reacting a conventional "soluble" enzyme (trypsin) with an inert base (agarose). This insoluble conjugate retains the activity of the original enzyme. Trypsin bound to agarose are highly stable and maintain denaturing conditions for longer time than the soluble trypsin.
The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

应用

A very active and very stable trypsin agarose derivative has been used to optimize the design of the synthesis of a model dipeptide, benzoylarginine leucinamide. Trypsin has also been used in a study to investigate protonation-state determination in proteins using high-resolution X-ray crystallography.
Trypsin Agarose has been used for enzymatic hydrolysis of prolamins and gliadin to generate peptides.

包装

50 units in glass bottle

其他说明

Insolubilized

单位定义

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 ℃ (titrimetric assay).

外形

Suspension in approx. 10 mM acetic acid, pH 3.2

基本信息

酶学委员会(EC)编号3.4.21.4 ( BRENDA | IUBMB )
MDL编号MFCD00082094
eCl@ss42020142
NACRESNA.54

产品性质

质量水平200
生物来源bovine pancreas (trypsin)
形式buffered aqueous suspension
浓度≥15 units/mL (packed gel)
标记范围≥15 units per mL packed gel
基质 cross-linked beaded agarose
运输wet ice
储存温度2-8℃

安全信息

象形图GHS07
警示用语:Warning
危险声明H315 - H317 - H319 - H335
预防措施声明P280 - P302 + P352 - P305 + P351 + P338
危险分类Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
靶器官Respiratory system
储存分类代码10 - Combustible liquids
WGKWGK 3
个人防护装备Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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