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Hybrisol® I Hybridization Solution, (50% Formamide, 6X SSC)

品牌
Millipore
货号
S4040
包装型号
规格纯度
参考价格
5456.32 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Hybrisol® Hybridization Solutions are pre-made, ready-to-use solutions optimized for sensitive single and multiple gene copy detection using membrane-based hybridization techniques. They are designed to give maximum signal and clean background for hybridizations performed on nylon and nitrocellulose membranes. Hybrisol Solution contains 10% Dextran Sulfate, 1% SDS, 50% Formamide, 6X SSC, Sheared DNA, and Modified Denhardt′s Solution.

应用

Before using, preheat at 37°C to 45°C for 10 - 20 minutes to dissolve precipitants and mix thoroughly. Use 1 ml for every 15 cm2 membrane for prehybridization and hybridization. Prehybridize for 60 minutes at 42°C to 45°C. Hybridize overnight at 45°C to 52°C. Prehybridization and hybridization temperatures may have to be determined empirically. Refer to the Nomograph located under the "Protocols" link at S4040 product specific page at www.chemicon.com for hybridization temperatures with various stringencies guidelines.
All

外形

Liquid Hybrisol I Solution contains 10% Dextran Sulfate, 1% SDS, 50% Formamide, 6X SSC, Sheared DNA, and Modified

Denhardt′s Solution.

储存及稳定性

Store at room temperature tightly sealed when not in use. Solution can be stored refrigerated however it should be warmed to at least room temperature prior to use dilution for best results. Precipitates may form when the solution is cooled.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
HYBRISOL is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160405

产品性质

质量水平100
包装pkg of 500 mL
manufacturer/tradenameChemicon®
technique(s)Northern blotting: suitable
Southern blotting: suitable
运输ambient

安全信息

象形图GHS08
警示用语:Danger
危险声明H350 - H360D - H373
预防措施声明P202 - P260 - P280 - P308 + P313 - P405 - P501
危险分类Carc. 1B - Repr. 1B - STOT RE 2 Oral
靶器官Blood
储存分类代码6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGKWGK 3

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