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CpG WIZ® Fragile X Amplification Kit

品牌
Sigma-Aldrich
货号
S7807
规格纯度
The components of the CpG WIZ Fragile X Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples.
参考价格
9446.8 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Methylation of cytosines located 5′ to guanosine is known to have a profound effect on the expression of several eukaryotic genes (Bird, 1992). In normal cells, methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG islands, remain unmethylated. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event in immortalized and transformed cells (Antequera, 1990) and has been associated with transcriptional inactivation of defined tumor suppressor genes in human cancers (Herman, 1994; Merlo, 1995). The Fragile X gene exhibits characteristic hypermethylation.



Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996 ). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.

应用

The components of the CpG WIZ Fragile X Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples.
Principles of the Technique

Use of either the CpGenome DNA Modification Kit (Cat. No. S7820) or the CpGENOME Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the CpG WIZ Fragile X Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.

组分

The components of the CpG WIZ Fragile X Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples with appropriate controls.

U Primer Set5 μM each primer (25X) 35 μL (neurtal cap) 90519 -15°C to -25°C

M Primer Set5 μM each primer (25X) 35 μL (red cap) 90520 -15°C to -25°C

W Primer Set5 μM each primer (25X) 35 μL (green cap) 90521 -15°C to -25°C

U control DNA0.1 μg/μL 50 μL (white cap) 90393 -15°C to -25°C

M control DNA0.1 μg/μL 50 μL (red cap) 90394 -15°C to -25°C

W control DNA0.05 μg/μL 50 μL (green cap) 90395 -15°C to -25°C

Universal 10X PCR Buffer 265 μL (blue cap) 90396 -15°C to -25°C

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
COPRIME is a registered trademark of Merck KGaA, Darmstadt, Germany
CPGWIZ is a trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32161000

产品性质

质量水平100
species reactivityhuman
manufacturer/tradenameCPGWIZ
Chemicon®
technique(s)PCR: suitable
NCBI登记号NM_002024.3
UniProt登记号Q06787
application(s)genomic analysis
运输dry ice
Gene Informationhuman ... FRAXA(2477)

安全信息

储存分类代码10 - Combustible liquids

Sigma-Aldrich

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