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Anti-trimethyl-Histone H4 (Lys20) Antibody, Trial Size, rabbit monoclonal

品牌
Sigma-Aldrich
货号
04-079-S
规格纯度
culture supernatant, Upstate®
参考价格
1660.9 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli, including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Histone H4 lysine 20 (H4K20) may undergo mono-, di-, or trimethylation, which is catalyzed by the methyltransferase PR-Set7 (Set8 or KMT5a). Methylated H4K20 plays a role in regulating DNA damage responses, mitosis, DNA replication, and gene expression. Trimethylation of H4K20 contributes to gene silencing, and is a mark of the repressive heterochromatin state.

特异性

Recognizes Histone H4 when trimethylated on Lys20.

免疫原

Peptide corresponding to human Histone H4 containing the sequence [HRmethKVL] on which lysine 20 is trimethylated.

应用

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 10 µL of either a negative control supernatant, or Anti-Trimethyl-Histone H4 (Lys20) antibody and the Magna ChIP A Kit (Cat. # 17-610). Successful immunoprecipitation of trimethyl-histone H4 (Lys20)-associated DNA fragments was verified by qPCR using ChIP Primers B-Globin. Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.

Dot Blot Analysis: AbSurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No 04-079-S, Anti-trimethyl Histone H4 (Lys20) (1:500 dilution). Proteins were visualized using a Donkey anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Chromatin Biology
Detect trimethyl-Histone H4 (Lys20) using this Anti-trimethyl-Histone H4 (Lys20) Antibody, rabbit monoclonal demontrated performance in WB, ChIP, & DB.

质量

Routinely evaluated by immunoblot.

目标描述

11kDa

外形

Rabbit monoclonal IgG cell culture supernatant in 0.1% sodium azide.

储存及稳定性

1 year at -20°C from date of shipment

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式culture supernatant
antibody product typeprimary antibodies
克隆monoclonal
species reactivityhuman, vertebrates
manufacturer/tradenameUpstate®
technique(s)ChIP: suitable
dot blot: suitable
western blot: suitable
同位素/亚型IgG
NCBI登记号NM_003538.3
UniProt登记号P62805
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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