产品说明
特异性
Recognizes the full length 32 kDa caspase-3 protein. Highly specific to caspase-3 and shows no cross-reaction with other caspase family members. Caspase-3 is ubiquitously expressed and like other caspases is synthesized as an inactive proenzyme. Upon activation, caspase-3 is cleaved generating two smaller subunits of 17 kDa and 12 kDa. Caspase-3 is one of the most extensively studied enzyme in apoptosis. Activation of caspase-3 occurs in response to a variety of apoptotic inducers.
免疫原
Recombinant full-length human caspase-3 protein
Epitope: large subunit & proform
应用
Western blot: 1:1000. The antibody will detect a band at 32 kDa corresponding to the inactive unprocessed form of caspase 3 as well as a 17-22 kDa band corresponding to the large subunit of active caspase 3.
Immunohistochemistry (frozen and paraffin): 1:500.
Optimal working dilutions must be determined by end user.
Research Sub Category
Caspases
Enzymes & Biochemistry
Research Category
Apoptosis & Cancer
Metabolism
Anti-Caspase 3 Antibody, large subunit & proform, clone 4-1-18 detects level of Caspase 3 & has been published & validated for use in WB, IH(P).
联系
Replaces: 04-439
外形
Purified immunoglobulin by protein A. Liquid in PBS, pH 7.6, with 0.1% sodium azide.
Format: Purified
储存及稳定性
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
分析说明
Control
POSITIVE CONTROL: 293 (human embryonic kidney) cell lysates
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 4-1-18, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
| 同位素/亚型 | IgG2aκ |
| NCBI登记号 | NM_004346.3 NM_032991.2 |
| UniProt登记号 | P42574 |
| 运输 | wet ice |
| Gene Information | human ... CASP3(836) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
