产品说明
一般描述
Calponin 1 is a 34 kDa protein that is predominantly found in smooth muscle tissue. It is a high-affinity actin-binding protein, but also interacts with tropomyosin, and calmodulin to modulate muscle contraction. When bound to F-actin, it inhibits the activity of the actin-activated myosin ATPase. Calponin 1 is regulated by reversible phosphorylation involving PKC and CAMKII kinases, and type 2A phosphatases. In non-muscle cells, calponin 1 may play a role in cell migration, motility, and proliferation.
特异性
This antibody recognizes the C-terminus of Calponin-1.
Other homologies: Rat (92% sequence homology) and Mouse (83% sequence homology).
免疫原
KLH-conjugated linear peptide corresponding to the C-terminus of human Calponin-1.
Epitope: C-terminus
应用
Research Category
Cell Structure
Immunohistochemistry Analysis: A 1:1,000 dilution from a reprsesentative lot detected Calponin-1 in human seminal vesicle tissue.
Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected Calponin-1 in smooth muscle cells from human seminal vesicle tissue.
This Anti-Calponin-1 Antibody is validated for use in Western Blotting, IHC(P), Immunofluorescence for the detection of Calponin-1.
Research Sub Category
Cytoskeleton
质量
Evaluated by Western Blot in human aortic smooth muscle tissue.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Calponin-1 in human aortic smooth muscle tissue.
目标描述
~33 kDa observed
联系
Replaces: 04-589
外形
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Human aortic smooth muscle tissue
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | human |
species reactivity (predicted by homology) | chimpanzee (based on 100% sequence homology), sheep (based on 100% sequence homology), bovine (based on 100% sequence homology) |
technique(s) | immunofluorescence: suitable immunohistochemistry: suitable (paraffin) western blot: suitable |
NCBI登记号 | NP_001290 |
UniProt登记号 | P51911 |
运输 | wet ice |
Gene Information | human ... CNN1(1264) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |