产品说明
一般描述
dTTP is a 100 mM clear, colorless solution of lithium salt (pH 7).
dTTP solution in water (pH 7), 100 mM, lithium salt.
应用
Use dTTP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.
It has been used in PCR fingrprinting and PCR techniques.
质量
Typical analysis:
- ≥98% dTTP (HPLC), ≤1.5% dTDP (HPLC), ≤0.5% dTMP (HPLC)
- Absorbance determination A250/A260, A280/A260, A290/A260
- DNase activity negative, according to the current Quality Control procedures
- Suitability for PCR
单位定义
1 μmol dTTP = 9.5 A260units
其他说明
For life science research only. Not for use in diagnostic procedures.
Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:
- nucleotide sets (all 4 nucleotides in separate vials)
- ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)
They are also vital components of:
- kits (dNTPs, buffer, and polymerase in separate vials)
- ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]
基本信息
经验(实验)分子式 | C10H16N2O14P3Na |
分子量 | 504.15 |
MDL编号 | MFCD00080338 |
PubChem化学物质编号 | 329749302 |
产品性质
质量水平 | 100 |
测定 | ≥98% |
形式 | solution |
用途 | sufficient for 6,250 standard reactions (PCR) |
包装 | pkg of 250 μL (25 μmol) |
manufacturer/tradename | Roche |
浓度 | 100 mM |
technique(s) | DNA amplification: suitable DNA sequencing: suitable |
运输 | dry ice |
储存温度 | −20℃ |
InChI | 1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);; |
InChI key | KAELIXTVSANDSE-UHFFFAOYSA-N |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | No data available |
闪点(C) | No data available |