产品说明
一般描述
Anti-Rab5 antibody, Mouse monoclonal (mouse IgG3 isotype) is derived from the hybridoma Rab5-65 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to C-terminal region of human Rab5, conjugated to KLH. Rab5 is ubiquitously expressed in human tissues. It localizes mainly to early endosomes, but is also present on the plasma membrane.
Rab5 (Ras-related protein Rab-5A) is a small GTPase , that has three isoforms - Rab5A, Rab5B and Rab5C. It is upregulated in several secretory cells. It is located on human chromosome 3p24.
免疫原
synthetic peptide corresponding to the C- terminal region of human Rab5, conjugated to KLH.
应用
Anti-Rab5 antibody, Mouse monoclonal has been used in:
- enzyme linked immunosorbent assay (ELISA)
- immunocytochemistry
- co-immunoprecipitation assay
- western blotting
- immunofluorescence
生化/生理作用
Among the proteins recruited by the GTP-bound active Rab5 are Rabaptin-5 and early endosome antigen 1(EEA1).
Rab5 (Ras-related protein Rab-5A) controls endosomal trafficking. Overexpression of Rab5A leads to metastasis and poor prediction in colorectal cancer patients. It modulates common Rab5 effectors to control the secretory pathways. It controls the homotypic integration between early endosomes and also the integration between endocytic vesicles and early endosomes.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD01322589 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | Rab5-65, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | ~24 kDa |
| species reactivity | cat, rat, mouse, human, bovine |
| 浓度 | ~2 mg/mL |
| technique(s) | immunocytochemistry: 5-10 μg/mL using HeLa cells or NIH-3T3 or NRK indirect ELISA: suitable microarray: suitable western blot: suitable |
| 同位素/亚型 | IgG3 |
| UniProt登记号 | P20339 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... RAB5A(5868) mouse ... Rab5a(271457) rat ... Rab5a(64633) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
