产品说明
一般描述
Rho-associated coiled-coil protein kinase 1 or ROCK-1 is a serine/threonine kinase that regulates actin assembly, cell movement and contraction. This kinase activates the JNK signaling pathway, modulates stress granule formation and regulates apoptosis in cardiac myocytes and stress granules .
免疫原
synthetic peptide corresponding to amino acids 1336-1354 located at the C-terminus of human ROCK-1, conjugated to KLH. This sequence is identical in mouse and rat ROCK-1.
应用
Anti-ROCK-1 antibody produced in rabbit has been used in western blotting and immunocytochemistry.
生化/生理作用
Rho-associated kinases (ROCK or Rho-kinase) is implicated in Rho-mediated actin reorganization such as formation of stress fibres and focal adhesions and smooth muscle contraction. Rho-kinases lead to an increase of myosin light chain (MLC) phosphorylation and thereby induce actomyosin based contractility. ROCK accumulates at the cleavage furrow during cytokinesis. In addition, a dominant negative form of ROCK increases multinuclei in mammalian cells. ROCK directly phosphorylates and activates LIM-kinase (LIMK), resulting in downstream phosphorylation and inaction of the actin depolymerizing factor or cofilin.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD02264068 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen 160 kDa |
| species reactivity | human, rat, mouse |
| technique(s) | immunoprecipitation (IP): 10-20 μL using of human acute T cell leukemia Jurkat cell line and of mouse fibroblasts NIH3T3 cell line microarray: suitable western blot: 1:1,000-1:2,000 using of human acute T cell leukemia Jurkat cell line and of mouse fibroblasts NIH3T3 cell line |
| UniProt登记号 | Q13464 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... ROCK1(6093) mouse ... Rock1(19877) rat ... Rock1(81762) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
