产品说明
一般描述
Ras-related protein Rab-14 (Rab14) is a member of the Rab11 subfamily of small guanosine triphosphatases (GTPases). It is associated with the early endosomal clathrin-coated trans‐Golgi network microdomain and is ubiquitously expressed. Rab14 belongs to the RAS oncogene family and is mapped to human chromosome 9q33.2.
特异性
Anti-Rab14 recognizes human, rat, and mouse Rab14 (not tested in other species).
应用
Anti-Rab14 antibody produced in rabbit has been used in western blotting and immunofluorescence.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
生化/生理作用
Ras-related protein Rab-14 (Rab14) is associated with the trafficking of the fibroblast growth factor receptor 2 (FGFR2) and regulates N-cadherin turnover. It is involved in membrane trafficking between the Golgi complex and endosomes. In polarized epithelial cells, Rab 14 regulates delivery of cargo from the trans‐Golgi network (TGN) to the apical membrane domain. Rab14 has also been implicated in phagosomal biogenesis. It acts as a tumor oncogene in non-small cell lung carcinoma (NSCLC) and is upregulated in gastric cancer.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
储存及稳定性
For continuous use, store at 2–8 ℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~24 kDa |
| species reactivity | rat, mouse, human |
| 浓度 | ~1.0 mg/mL |
| technique(s) | indirect immunofluorescence: 2.5-5 μg/mL using rat NRK cells fixed and permeabilized with 4% paraformaldehyde followed by 0.4% saponin western blot: 2-4 μg/mL using whole extracts of mouse brain and human Raji cells |
| UniProt登记号 | P61106 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... RAB14(51552) mouse ... Rab14(68365) rat ... Rab14(94197) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
