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Restorase® DNA Polymerase with 10× Reaction Buffer

品牌
Sigma-Aldrich
货号
R1028
规格纯度
Enzyme blend for PCR amplification of damaged DNA
参考价格
3278.59 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Restorase DNA Polymerase with 10× Reaction Buffer combines Sigma′s long and accurate enzyme technology with a small amount of DNA repair enzyme. The optimized blend will initiate the repair and further amplification of damaged DNA templates greater than 800 bp. Restorase has also been shown to increase yield on undamaged DNA templates.

应用

Restorase® DNA Polymerase with 10× Reaction Buffer has been used in DNA repair.

生化/生理作用

DNA templates are often damaged on exposure to heat, acids, alkylating agents, and light. These damaged templates result in inefficient or failed DNA amplification by polymerase chain reaction (PCR). Restorase® DNA Polymerase repairs the damaged sites on the DNA templates and initiates subsequent template copying. Restorase treatment of the DNA depends on the level of template damage.

特点和优势

  • Reliable amplification of damaged DNA
  • When thermostable polymerases fail, this enzyme amplifies the sequence efficiently
  • Efficient amplification of sequences in multiplex reactions
  • Increased yield and amplicon specificity
  • Amplifies a broad spectrum of amplicon sizes varying from 200 bp to 20 kb
  • Can repair 3′ bungs, nicks, and abasic sites

包装

The enzyme is provided with an optimized 10× reaction buffer provided as 1 vial/250 units.

其他说明

View more detailed information on Restorase DNA Polymerase at www.sigma-aldrich.com/restorase.
Learn more about our offering of specialty enzymes at www.sigma-aldrich.com/specialtyenzymes.

法律信息

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护:5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品,即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可,即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利,包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途,需另外征得Roche许可。有关购买许可的更多信息,可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
Restorase is a registered trademark of sigma-aldrich Co. LLC

基本信息

NACRESNA.55

产品性质

质量水平200
形式liquid
用途 sufficient for 50 reactions
特点Long & Accurate PCR
dNTPs included: no
hotstart: no
浓度2.5 units/μL
technique(s)PCR: suitable
颜色 colorless
input purified DNA
运输wet ice
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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