产品说明
一般描述
v-akt murine thymoma viral oncogene homolog 1 (AKT1) is a Ser/Thr protein kinase, and is also called protein kinase B (PKB). This protein is activated when it is phosphorylated at serine 473 and threonine 308. This gene is localized on human chromosome 14q32.32.
生化/生理作用
v-akt murine thymoma viral oncogene homolog 1 (AKT1) is responsible for the phosphorylation and cytoplasmic localization of CKDN1A (cyclin-dependent kinase (CDK) inhibitor 1, also referred to as p21). However, p21 is one of the CDKNs which normally resides in the nucleus and its cytoplasmic localization, mediated by AKT1, is common to various cancers where it suppresses proteins essential for apoptosis. Studies show that up-regulation of AKT1 serves as a poor prognostic marker in breast cancer, which however, depends upon factors including population, hormone receptor status, and hormonal or trastuzumab therapy status.
外形
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
制备说明
after opening, aliquot into smaller quantities and store at -70 ℃. Avoid repeating handling and multiple freeze/thaw cycles
法律信息
PRECISIO is a registered trademark of sigma-aldrich Co. LLC
基本信息
| NACRES | NA.32 |
产品性质
| 重组 | expressed in baculovirus infected Sf9 cells |
| 产品线 | PRECISIO® Kinase |
| 测定 | ≥70% (SDS-PAGE) |
| 形式 | buffered aqueous glycerol solution |
| specific activity | 103-139 nmol/min·mg |
| 分子量 | ~85 kDa |
| NCBI登记号 | NM_005163 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... AKT1(207) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
