产品说明
特异性
Specific for Dopamine Receptor D4. Mouse Dopamine receptor D4 is a 387 aa, G-protein coupled and transmembrane receptor protein (Van Tol et al. 1992; Legerski & Peterson 1992; Livingstone et al. 1992; Seeman et al. 1994). The immunogen peptide shows no significant homology with other dopamine receptors (D1, D2, D3 or D5).
SPECIES REACTIVITIES: The immunogen peptide shows sequence homology of 90% with rat and human D4 receptors (Van Tol et al. 1992; Legerski & Peterson 1992; Livingstone et al. 1992; Seeman et al. 1994; O′Malley et al. 1992). Antibody cross-reactivity with D4R from various species is not known.
免疫原
Epitope: extracellular domain
A 10 amino acid peptide sequence from the mouse D4 receptor within the 3rd extracellular domain.
应用
Research Category
Neuroscience
Western blot: 1-10 μg/mL (Chemiluminescence technique). Approximately 100-400 μg of membrane protein/lane should be loaded to visualize the receptor band. Antibody detects ~55kDa band in HT222 cells and mouse hippocampal membranes. {http://www.jneurosci.org/cgi/reprint/21/16/6069.pdf, Ishige, K et.al.(2001) J. Neuroscience 21(16):6069-6076.
ELISA: 0.5-1.0 μg/mL (1 μg/mL immunogen peptide/well)
Not tested in immunohistochemical methods.
Optimal working dilutions must be determined by the end user.
Research Sub Category
Neurotransmitters & Receptors
Anti-Dopamine D4 Receptor Antibody, extracellular domain is an antibody against Dopamine D4 Receptor for use in ELISA & WB.
联系
Replaces: AB1789P
外形
Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA
储存及稳定性
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | mouse |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable western blot: suitable |
NCBI登记号 | NM_000797.2 |
UniProt登记号 | P21917 |
运输 | dry ice |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |