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Human Laminin (pepsinized) Purified Protein

品牌
Sigma-Aldrich
货号
AG56P
包装型号
规格纯度
参考价格
5367.5 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

AG56P contains a mixture of human laminins containing the beta1 chain, principally laminin 1, 2, 3, 6, 8 & 10. This preparation is immunologically and biologically identical to intact human laminin. AG56P is suitable for use in ELISA, production of antiserum, cell adhesion and attachment assays, and neurite-stimulation assays. This pepsinized laminin preparation runs as twin bands migrating at approximately 160kDa and 130kDa by SDS-PAGE under reducing conditions.

Preparation: Chemicon′s purified human laminin is prepared from freshly frozen human placenta tissue that is homogenized in PBS with a blender and the mixture centrifuged. The pellet is then washed with 0.5M acetic acid, collected and subjected to and extended Pepsin A digestion. The pepsin digest is neutralized, centrifuged to clear debris, and applied to an antibody column containing mouse anti-human laminin monoclonal 4E10 monoclonal antibody {MAB1921}. The solubilized laminin solution is passed over the column, and utlimately the laminin is released via acidification with KSCN. Then the material is dialyzed against PBS. The antibody 4E10 is specific for laminin beta1 chain. This chain is found in Laminin′s 1,2,3,6,8,10 under the native conditions used to purify the material.
Product Source: Human placenta, tested negative for hepatitis B virus, hepatitis C virus (HCV), HIV-1, HIV-2, HTLV-1, HTLV-2, and Treponema pallidum. Handle as if potentially infectious.

质量

Each lot is analyzed on SDS-PAGE for band integrity and expected sizes prior to packaging and release

外形

Purified laminin protein in liquid in Tris Buffer Saline with 0.01% sodium azide.

制备说明

Purified from a mild pepsin digestion followed by affinity chromatography on monoclonal (4E10) anti-human laminin-Sepharose.

储存及稳定性

Store at -20°C or -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a table-top centrifuge to dislodge any liquid in the container′s cap.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160405
NACRESNA.75

产品性质

质量水平100
生物来源human
测定≥95% (SDS-PAGE)
形式liquid
manufacturer/tradenameChemicon®
浓度0.25 mg/mL
technique(s)cell culture | mammalian: suitable
inputsample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type hematopoietic stem cell(s)
sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
NCBI登记号NM_002291.1
UniProt登记号P07942
运输dry ice
储存温度−20℃
−70℃
Gene Informationhuman ... LAMB1(3912)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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