产品说明
特异性
This antibody may also react with mouse because of extremely high sequence conservation in the SEM3F protein.
Recognizes Semaphorin 3F (Sema3F), recombinant and purified protein. By Western blot the antibody recognizes a protein of 90 kDa in total extracts from tissue culture cells producing Sema3F. Semaphorin 3F is expressed ubiquitously with higher levels in lung, brain, spleen, muscle and heart.
免疫原
Synthetic peptide from human Semaphorin 3F (Catalog number AG363). (TPWTQTQAVRFRFSRATDGALRPM).
应用
Research Category
Neuroscience
Anti-Semaphorin 3F Antibody is an antibody against Semaphorin 3F for use in IC & WB.
Research Sub Category
Growth Cones & Axon Guidance
Western blot: 0.5-1.0 μg/mL using ECL. Suggested antibody dilution buffer is TBST (10 mM Tris-HCl, pH 8.0, 150 mM NaCl with 0.05% Tween 20). Suggested incubation is for two hours at room temperature or overnight at 2-8°C.
Immunocytochemistry.
Optimal working dilutions must be determined by the end user.
目标描述
90 kDa
联系
Replaces: AB5471
外形
ImmunoAffinity Purified
Affinity Purified immunoglobulin. Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.
储存及稳定性
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析说明
Control
Embryonic brain tissue, neural growth cones
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 纯化方式 | affinity chromatography |
| species reactivity | rat, human |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunocytochemistry: suitable western blot: suitable |
| NCBI登记号 | NM_004186.2 |
| UniProt登记号 | Q13275 |
| 运输 | wet ice |
| Gene Information | human ... SEMA3F(6405) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
