位置:首页 > 品牌 > Sigma-Aldrich >

pT7-FLAGTM-2 Expression Vector

品牌
Sigma-Aldrich
货号
P1243
包装型号
规格纯度
参考价格
4843.68 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

pT7-FLAG-2 is a 4.8 kb E . coli expression vector used for cloning and cytoplasmic expression of a properly inserted open reading frame as a C-terminal FLAG® fusion protein containing the FLAG epitope (DYKDDDDK). The promoter region of the very strong phage T7 promoter drives transcription of ORFFLAG fusion constructs. This vector requires the use of E. coli cells containing a source of the T7 polymerase, such as BL21(DE3)T1R cells, Catalog Number B2935.

The C-terminal FLAG fusion protein may be detected using Monoclonal ANTI-FLAG?M2, Catalog Number
F3165, and purified using the ANTI-FLAG M2 Affinity Gel, Catalog Number A2220.

Vector Maps and Sequences

组分

  • pT7-FLAG-2 Expression Vector, 10 μg (P6867) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
  • pT7-FLAG-2-BAP Control Vector 1 μg ( P7117) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

法律信息

FLAG is a registered trademark of sigma-aldrich Co. LLC
pT7-FLAG is a trademark of sigma-aldrich Co. LLC

基本信息

NACRESNA.85

产品性质

质量水平200
等级for molecular biology
运输dry ice
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号