产品说明
一般描述
PhotoClick Cholesterol is a clickable, photoreactive sterol probe for use in combination with quantitative mass spectrometry to globally map cholesterol-protein interactions directly in living cells. In addition to the UV-activatable cross-linking diazirine group that reacts with proteins, the sterol contains an alkyne group for incorporation of reporter and affinity tags through click-chemistry.
PhotoClick Cholesterol is a synthetic analog of cholesterol and is photoreactive. It comprises the alkyne group.
应用
PhotoClick Cholesterol has been used:
- in lysosome-associated membrane protein 1 (LAMP1) crosslinking studies
- as an analog of cholesterol in binding and competition assay of sterol binding proteins from yeast
- as a conjugate with the ectodomain of lysosomal integral membrane protein-2 (LIMP-2)
生化/生理作用
PhotoClick Cholesterol serves as a probe for tracking cholesterol-binding proteins. The copper-catalyzed azide-alkyne cycloaddition is the basis for its probe functionality. The alkyne group is essential for the click chemistry property. Its binding to partners is mediated by photocrosslinking.
包装
2 mL Amber Serum Vial with Stopper and Crimp Cap (700147P-250ug)
2 mL Amber Serum Vial with Stopper and Crimp Cap (700147P-100ug)
基本信息
| 经验(实验)分子式 | C30H46N2O3 |
| 分子量 | 482.70 |
| NACRES | NA.25 |
产品性质
| 测定 | >99% (TLC) |
| 形式 | powder |
| 包装 | pkg of 1 × 100 μg (with stopper and crimp cap (700147P-100ug)) pkg of 1 × 250 μg (with stopper and crimp cap (700147P-250ug)) |
| manufacturer/tradename | Avanti Polar Lipids 700147P |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
