产品说明
一般描述
Protein inhibitor of activated STAT protein 3 (PIAS3) is an inhibitor of activated STAT3. The gene encodes for a 619-amino acid protein and has a molecular mass of 68 kDa. PIAS3 shares 55% identity with PIAS1 and PIASX proteins and 39% identity with PIASY. PIAS3 is widely expressed in various human tissues.
免疫原
synthetic peptide corresponding to amino acids 591-604 of human PIAS3.
应用
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Monoclonal Anti-PIAS3 antibody produced in mouse has been used in:
- enzyme linked immuno sorbent assay (ELISA)
- immunoblotting
- immunocytochemistry
生化/生理作用
Protein inhibitor of activated STAT protein 3 (PIAS3) and other members of this family can act as small ubiquitin-like modifier (SUMO) E3 ligases and interfere with the transcription activity of many transcription factors. PIAS3 binds to microphthalmia-associated transcription factor (MITF), a key DNA-binding protein. It blocks MITF DNA-binding activity and co-expression of MITF. PIAS3 in NIH 3T3 cells inhibits MITF-driven transcription activity.
目标描述
PIAS3 encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD09265430 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | PIA3, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~68 kDa (additional band at 44 kDa may be present) |
| species reactivity | mouse, chicken, bovine, human, canine |
| 浓度 | ~2 mg/mL |
| technique(s) | immunocytochemistry: suitable indirect ELISA: suitable western blot: 2-4 μg/mL using HepG2 total cell extract |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | Q9Y6X2 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... PIAS3(10401) mouse ... Pias3(229615) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
