产品说明
应用
Pyruvate is critical for gluconeogenesis, lipogenesis, glyceroneogenesis, neurotransmitter biosynthesis and glucose-induced insulin, and is used to study these processes.
The enzyme from Sigma has been used as a positive control during the assay of pyruvate carboxylase activity in cell-free extracts of Corynebacterium glutamicum.
生化/生理作用
Pyruvate carboxylase catalyzes the carboxylation of pyruvate to oxaloacetate. Pyruvate carboxylase is a mitochondrial protein that has a biotin prosthetic group that requiries magnesium or manganese and acetyl CoA.
单位定义
One unit will convert 1.0 μmole of pyruvate and CO2 to oxalacetate per min at pH 7.8 at 30 ℃.
外形
Solution in 50% glycerol containing 0.05 M Tris-HCl, pH 7.4, 2 mM magnesium acetate and 1 mM EDTA.
制备说明
亲和纯化
基本信息
| 酶学委员会(EC)编号 | 6.4.1.1 ( BRENDA | IUBMB ) |
| MDL编号 | MFCD00132143 |
| NACRES | NA.26 |
产品性质
| 形式 | buffered aqueous glycerol solution |
| 质量水平 | 200 |
| specific activity | 5-25 units/mg protein (BCA) |
| 浓度 | ≥0.5 mg/mL |
| 异质活性 | lactic dehydrogenase ≤0.5% |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
