产品说明
一般描述
Monoclonal Anti-Prox1 (mouse IgG2b isotype) is derived from the hybridoma 4G10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant fusion protein of human Prox1. Prospero related homeobox gene 1 (Prox1) belongs to transcription factor subfamily and is homologous to Drosophila homeobox protein, prospero.
免疫原
recombinant fusion protein corresponding to amino acids 547-737 of human Prox1.
应用
Anti-Prox1 antibody, Mouse monoclonal has been used in various immunochemical techniques.
生化/生理作用
Prospero related homeobox gene 1 (Prox1) is a key regulator for neuroblast fate determination and photoreceptor development. Prox1 regulates cell differentiation and organogenesis in lens, retina, lymphatic vessels, liver and pancreas. It was found to act as a tumor suppressor gene, because its deficiency or its mutated forms have been implicated in various human cancers. Knockdown of Prox1 by siRNA or by RNA mutations significantly accelerated the in vitro and in vivo growth of hepatocellular carcinoma (HCC) as well as esophageal cancer cells. Prox1 is hypermethylated and transcriptionally silenced in primary and metastatic breast cancer.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD09039204 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | 4G10, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~84 kDa |
| species reactivity | human, lizard, rat, chicken |
| 浓度 | ~1.5 mg/mL |
| technique(s) | immunocytochemistry: suitable indirect ELISA: suitable indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed HepG2 cells western blot: suitable |
| 同位素/亚型 | IgG2b |
| UniProt登记号 | Q92786 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... PROX1(5629) rat ... Prox1(305066) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
