产品说明
一般描述
Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. This enzyme is in a recombinant form, expressed in E. coli. Taq DNA polymerase enzyme can withstand high temperatures up to 95 ℃ without significant loss of activity. Taq DNA Polymerase is a magnesium ion-dependent enzyme. Taq DNA Polymerase possesses 5′→3′ DNA polymerase activity and 5′→3′ exonuclease activity.
应用
CORET PCR kit has been used for the amplification of genomic DNA during the study of genetic diversity in various species of Manilkara zapota.
PCR Core kit with Taq DNA polymerase is suitable for the amplification of genomic DNA, cDNA, or cloned DNA templates. Each component has been tested in the polymerase chain reaction. Optimal concentrations of Taq polymerase, template DNA, primers, and MgCl2 will depend on the specifics of the system. It may be necessary to optimize each component separately, especially the Taq and Mg2+ concentrations and the cycling parameters.
数量
试剂盒中含有足够用于250单位Taq DNA聚合酶的试剂。
其他说明
以下试剂必需但并未提供在试剂盒内：
- 矿物油，M 8662(可选)
- 热循环仪
- 引物
- 待扩增的DNA
- 氯仿，C 7559(可选)
法律信息
No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
基本信息
| eCl@ss | 32161000 |
| NACRES | NA.55 |
产品性质
| 质量水平 | 200 |
| 形式 | liquid |
| 用途 | sufficient for 250 reactions |
| 特点 | dNTPs included hotstart: no |
| technique(s) | PCR: suitable |
| 颜色 | colorless |
| input | purified DNA |
| 运输 | wet ice |
| 储存温度 | −20℃ |
组份列表
组份可单独销售
| 货号 | 组份 |
| P2192 | 10 |
| P2317 | 10 |
| M8787 | Magnesium chloride solution, PCR Reagent, 25 mM MgCI2 solution for PCR 1 mL/vial |
| W1754 | Water, PCR Reagent 3 vial(s) |
| D7295 | Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent .25 mL |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
