产品说明
一般描述
Nuclear pore complexes (NPCs) are aqueous channels that impart access to the nucleus and modulate the transport of RNA, proteins and ribosome subunits across the nuclear envelope. Nucleoporins, which are conserved proteins, form a complex network and generate these channels. Each NPC is a large, complex cylindrical structure consisting of around 500 nucleoporins.
Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma 414 produced by the fusion of mouse myeloma cells (NS-1 cells) and splenocytes from BALB/c mice immunized with rat liver nuclei extract. The nuclear pore complexes (NPCs) are structurally highly conserved in all eukaryotic cells. They contain 30-40 different proteins resulting in a complex with a molecular weight of ~50 kDa.
免疫原
rat liver nuclei extract.
应用
Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal has been used for immunohistochemistry.
Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal has been used in:
- immunoblotting
- immunoprecipitation
- immunocytochemistry
- immunoelectron microscopy
- immunofluorescence
生化/生理作用
Nucleocytoplasmic exchanges occur through nuclear pore complexes (NPCs) that are circular apertures in the nuclear envelope where the inner and the outer nuclear membrane are joined. Through the NPC there is a passive transport of small molecules (ions and water) that are smaller than 9-10 nm. Large molecules such as RNA and proteins have to be actively transported through the NPC in an energy and signal dependent manner. Many proteins interact with members of the NPC complex.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD02263844 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 414, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen 62-210 kDa |
| species reactivity | Xenopus, yeast, human, rat, mouse |
| 浓度 | ~3 mg/mL |
| technique(s) | electron microscopy: suitable immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: 2-4 μg/mL using HeLa total cell extract |
| 同位素/亚型 | IgG1 |
| 运输 | dry ice |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
