产品说明
一般描述
Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.
应用
4-Nitrophenyl phosphate disodium salt hexahydrate has been used in enzyme-linked immunosorbent assay (ELISA) as a substrate for streptavidin-Alkaline Phosphatase. It has also been used as a substrate in control assay for comparing 4-aminophenyl phosphate/diethanolamine (APP/DEA) assay for alkaline phosphatase.
生化/生理作用
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol. pNPP is a common chromogenic substrate for alkaline phosphatase.
数量
Contains 15 mg substrate per tablet
基本信息
| 线性分子式 | O2NC6H4OP(O)(ONa)2 · 6H2O |
| 分子量 | 371.14 |
| Beilstein | 5324661 |
| EC 号 | 224-246-5 |
| MDL编号 | MFCD00066288 |
| PubChem化学物质编号 | 24897580 |
| NACRES | NA.83 |
产品性质
| 形式 | tablet |
| 质量水平 | 200 |
| 溶解性 | water: 1 tablet/3 mL, clear, colorless |
| 储存温度 | −20℃ |
| SMILES string | O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1 |
| InChI | 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2 |
| InChI key | KAKKHKRHCKCAGH-UHFFFAOYSA-L |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
