Anti-PAXIP1

产品说明

一般描述

PAX-interacting protein 1 (UniProt: Q6NZQ4; also known as PAX transactivation activation domain-interacting protein, PAXIP1) is encoded by the Paxip1 (also known as Ptip) gene (Gene ID: 55982) in murine species. PAXIP1 is a nuclear matrix protein that is involved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes such as the MLL2/MLL3 complex. In vitro it is shown to be involved in the homologous recombination mechanism for the repair of double-strand breaks. It recruits Tp53bp1 to DNA damage foci and effective DNA damage response appears to require the association with Tp53bp1 phosphorylated by ATM. It also recruits PAGR1A (Pax-Interacting Protein 1-associated glutamate rich protein 1a) to DNA damage sites and PAGR1A:PAXIP1 complex is believed to be essential for cell survival. PAXIP1 expression is reported in all tissues and high expression is observed in embryonic kidney and brain. PAXIP1 contains 6 BRCT domains and BRCT domains 1 and 2 mediate its interaction with PAGR1A and interaction with MLL2/MLL3 is accomplished with BRCT domains 5 and 6. Mice with defective Paxip1 gene are developmentally retarded and usually face embryonic lethality by day 9.5 (E9.5).

特异性

This rabbit polyclonal antibody detects PAX-interacting protein 1 in human and murine cells. It targets an epitope within 199 amino acids from the N-terminal region.

免疫原

His-tagged recombinant fragment corresponding to 199 amino acids from the N-terminal region of murine PAX-interacting protein 1.

Epitope: unknown

应用

Chromatin Immunoprecipitation Analysis: A representative lot detected PAXIP1 in mouse embryonic fibroblasts (Cho, Y.W., et. al. (2009). Cell Metab. 10(1):27-39).

ChIP-seq Analysis: Analysis: A representative lot detected PAXIP1 in ChIP-seq applications (Callen, E., et. al. (2012). Immunity. 37(6):971-85).

Immunoprecipitation Analysis: A representative lot detected PAXIP1 in Immunoprecipitation applications (Gong, Z., et. al. (2009). J Biol Chem. 284(11):7284-93).

Immunoprecipitation Analysis: 1 µg from a representative lot detected PAXIP1 in nuclear extract of wild type and PTIP KO mouse embryonic fibroblasts (Courtesy of Dr. Ji-Eun Lee and Dr. Kai Ge at NIH).

Western Blotting Analysis: A representative lot detected PAXIP1 in Western Blotting applications (Cho, Y.W., et. al. (2009). Cell Metab. 10(1):27-39; Gong, Z., et. al. (2009). J Biol Chem. 284(11):7284-93).

Anti-PAXIP1, Cat. No. ABE1877, is a highly specific rabbit polyclonal antibody that targets PAX-interacting protein 1 and has been tested for use in ChIP-seq and Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting.
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting with with recombinant Pax-interacting protein 1.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant PAX-interacting protein 1.

目标描述

~51 kDa observed (recombinant); 119.27 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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