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Anti-methylated TLS/FUS Antibody, clone 9G6

品牌
Sigma-Aldrich
货号
MABE463M
规格纯度
clone 9G6, from rat
参考价格
1415.25 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

RNA-binding protein FUS (UniProt: also known as 75 kDa DNA-pairing protein, Oncogene FUS, Oncogene TLS, POMp75, Translocated in liposarcoma protein) is encoded by the FUS (also known as TLS) gene (Gene ID: 2521) in human. Fused in sarcoma/translocated in liposarcoma FUS/TLS is a multi-functional nuclear protein that binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in super helical double-stranded DNA. It is involved in a variety of cellular functions including transcription, protein translation, RNA splicing, and transport and may play a role in maintenance of genomic integrity. A chromosomal aberration involving FUS is shown to cause of acute myeloid leukemia (AML). Mutations in FUS gene have been linked to Amyotrophic lateral sclerosis 6, with or without frontotemporal dementia that affects upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. (Ref.: Lagier-Tourenne, C., et al. (2010). Hum. Mol. Genet. 19(1); R46-R64).

特异性

Clone 9G6 detects methylated RNA-binding protein FUS in human cells. It targets an epitope with in 31 amino acids from the C-terminal region.

免疫原

Ovalbumin-conjugated linear peptide corresponding to 31 amino acids from the C-terminal region corresponding to the RGG3 domain of FUS (with all arginines methylated).

应用

Western Blotting Analysis: 0.05 ug/mL frrom a representative lot detected methylated TLS/FUS in 10 µg of HeLa cell lysate.

Western Blotting Analysis: 0.05 ug/mL frrom a representative lot detected methylated TLS/FUS in 10 µg of lysate from HeLa cells pretreated with AdOx. (Dormann, D., et al. (2012). The EMBO J. 31(22):4258-75)

Western Blotting Analysis: A representative lot detected methylated TLS/FUS in Western Blotting applications (Suárez-Calvet, M., et. al. (2016). Acta Neuropathol. 131(4):587-604).



Anti-methylated TLS/FUS, clone 9G6, Cat. No. MABE463, is a highly specific rat monoclonal antibody that targets methylated RNA-binding protein FUS and has been tested in Western Blotting.
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.05 ug/mL of this antibody detected methylated TLS/FUS in 10 µg of HepG2 cell lysate.

目标描述

~70 kDa observed; 53.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

质量水平100
生物来源rat
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆9G6, monoclonal
species reactivityhuman
包装antibody small pack of 25 μg
technique(s)western blot: suitable
同位素/亚型IgG2bκ
NCBI登记号NP_001164105
UniProt登记号P35637
Gene Informationhuman ... FUS(2521)

Sigma-Aldrich

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