位置:首页 > 品牌 > Sigma-Aldrich >

Anti-Cas9 (Chicken Polyclonal)

品牌
Sigma-Aldrich
货号
AB356492M
包装型号
规格纯度
from chicken
参考价格
5352.52 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

CRISPR-associated endonuclease Cas9 (UniProt: J7RUA5; also known as SaCas9, Cas9) is encoded by the Cas9 gene in Streptococcus aureus. Cas9 belongs to subtype II-A subfamily with one HNH Cas9-type domain. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. Cas9 has a bi-lobed architecture with a recognition lobe (residues 41-425) and a discontinuous nuclease lobe (NUC, residues 1-40 and 453-1053); the crRNA-target DNA lies in a channel between the 2 lobes. The NUC lobe has 2 endonuclease domains. The discontinuous RuvC-like domain in NUC cleaves the target DNA non-complementary to crRNA while the HNH nuclease domain cleaves the target DNA complementary to crRNA. Cas9 serves as a RNA-guided DNA endonuclease that cleaves double-stranded DNA targets with a protospacer adjacent motif (PAM) and complementarity to the guide RNA. The homologous region of the Streptococcus pyogenes is not closely related in amino acid sequence in S. aureus, hence this antibody does not recognize that protein.

特异性

This chicken polyclonal antibody detects Cas9 from Staphylococcus aureus. It targets an epitope within 251 amino acids from the C-terminal end. This antibody does not react with Cas9 from Streptococcus pyogenes.

免疫原

Epitope: C-terminus
His-tagged recombinant fragment corresponding to 251 amino acids from the C-terminal end of CRISPR-associated endonuclease Cas9 from Staphylococcus aureus.

应用

Anti-Cas9 (Chicken Polyclonal), Cat. No. AB356492, is a highly specific chicken polyclonal antibody that targets CRISPR-associated endonuclease Cas9 and has been tested in Immunofluorescence and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Cas9 in HEK293 cells transfceted with GFP-Cas9 from Staphylococcus aureus (Courtesy of Dr. Gerry Shaw).

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Cas9 in HEK293 cells transfceted with GFP-Cas9 from Staphylococcus aureus (Courtesy of Dr. Gerry Shaw).

质量

Evaluated by Western Blotting in HEK293 cells transfected with GFP-Cas9 from Staphylococcus aureus.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Cas9 in 40 µg of lysate from HEK293 cells transfected with GFP-Cas9 from Staphylococcus aureus.

目标描述

~57 kDa observed. Uncharacterized bands may be observed in some lysate(s).

外形

Format: Unpurified
Unpurified
Chicken polyclonal antibody in PBS with 0.02% sodium azide.

储存及稳定性

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

生物来源chicken
质量水平100
抗体形式unpurified
antibody product typeprimary antibodies
克隆polyclonal
species reactivitybacteria, Staphylococcus aureus
technique(s)immunofluorescence: suitable
western blot: suitable
同位素/亚型IgY
UniProt登记号J7RUA5

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号