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Anti-Calnexin Antibody, clone AF8

品牌
Sigma-Aldrich
货号
MABF2067M
规格纯度
clone AF8, from mouse
参考价格
5016.79 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Calnexin (UniProt: P27824; also known as IP90, Major histocompatibility complex class I antigen-binding protein p88, p90) is encoded by the CANX gene (Gene ID: 821) in human. Calnexin is a well-conserved, calcium binding, endoplasmic reticulum (ER) resident chaperone protein that associates with newly synthesized, incompletely folded monomeric glycoproteins and promotes their correct folding prior to secretion. It is also reported to target misfolded proteins for degradation. It is anchored to the ER membrane by a transmembrane domain. Calnexin associates with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes and may, therefore, function as a signaling complex regulating thymocyte maturation. Calnexin is synthesized with a signal peptide of 20 amino acids, which is cleaved to generate the mature form. Three isoforms of calnexin have been described that are generated by alternative splicing. Calnexin is shown to be phosphorylated at Ser564 by MAPK3/ERK1, which increases its association with ribosomes.

特异性

Clone AF8 detects Calnexin in human cells.

免疫原

TCR complexes isolated from the human T-cell leukemia line, DND41.

应用

Anti-Calnexin, clone AF8, Cat. No. MABF2067, is a mouse monoclonal antibody that detects calnexin in human cells and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Flow Cytometry Analysis: 1 µg from a representative lot detected Calnexin in one million MCF7 cells.

Immunoprecipitation Analysis: A representative lot detected Calnexin in Immunoprecipitation applications (Hochstenbach, F., et. al. (1992). Proc Natl Acad Sci USA. 89(10):4734-8; Rajagopalan, S., et. al. (1994). J Exp Med. 180(1):407-12).

Immunohistochemistry Analysis: A representative lot detected Calnexin in Immunohistochemistry applications (Hochstenbach, F., et. al. (1992). Proc Natl Acad Sci USA. 89(10):4734-8).

Flow Cytometry Analysis: A representative lot detected Calnexin in Flow Cytometry applications (Rajagopalan, S., et. al. (1994). J Exp Med. 180(1):407-12).

Western Blotting Analysis: A representative lot detected Calnexin in Western Blotting applications (Hochstenbach, F., et. al. (1992). Proc Natl Acad Sci USA. 89(10):4734-8).

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Calnexin in HeLa, A431 and Huvec cell lines.

Research Category
Inflammation & Immunology

质量

Evaluated by Immunocytochemistry in MCF7 cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Calnexin in MCF7 cells.

目标描述

67.57 kDa calculated.

联系

Replaces: MAB3126

外形

Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆AF8, monoclonal
species reactivityhuman
包装antibody small pack of 25 μg
technique(s)flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG1κ
NCBI登记号NP_001019820.1
UniProt登记号P27824
Gene Informationhuman ... CANX(821)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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