产品说明
一般描述
Monoclonal Anti-Mus81 (mouse IgG2a isotype) is derived from the hybridoma MTA30 2G10/3 produced by the fusion of mouse myeloma cells (Sp2/0 Ag.14 cells) and splenocytes from BALB/c mice immunized with Mus81 protein. Crossover junction endonuclease Mus81 protein was identified by screening a human cerebellum cDNA library with a probe from the yeast Mus81. The human Mus81 has 551 amino acids with a molecular mass of 59 kDa. The highest sequence similarity between different species is around the VERKX3D motif. This motif is conserved in the endonuclease family (XPF) family of nucleases. The protein has helix-hairpin-helix DNA-binding domains in the N- and C-termini of the protein that are also conserved.
免疫原
Mus81 protein.
应用
Anti-Mus81 antibody, Mouse monoclonal has been used in immunofluorescence, immunoblotting and immunoprecipitation.
生化/生理作用
Crossover junction endonuclease Mus81 is elevated in cells exposed to agents that block DNA replication. Knockout mice that are homozygous Mus81-/-, or their heterozygous cohorts Mus81-/+, are susceptible to spontaneous chromosomal damage and cancer predisposition suggesting that this protein is important for genomic integrity and tumor suppression
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD08705592 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | MTA30 2G10/3, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~59 kDa |
| species reactivity | human, monkey |
| technique(s) | immunoprecipitation (IP): suitable western blot: 0.5-1 μg/mL using HEK-293T total cell extract |
| 同位素/亚型 | IgG2a |
| UniProt登记号 | Q96NY9 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... MUS81(80198) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
