产品说明
一般描述
Monoclonal Anti-MRP1 (mouse IgG1 isotype) is derived from the QCRL-1 hybridoma produced by the fusion of mouse myeloma cells (SP2/O) and splenocytes from mice immunized with non-denatured membrane preparations of human H69AR small cell lung cancer cell line, which highly expresses MRP1.
Multidrug resistance associated protein 1 (MRP1) is present in subcellular vesicles and is composed of 1531 amino acids. It has a molecular weight of 190kDa. The gene encoding MRP1 is localized in human chromosome 16p13.11.
特异性
Does not cross-react with the human MDR1 and MDR3, mouse MRP1, and human MRP2, MRP3, MRP4, MRP5, and MRP6 gene products. The antibody may cross-react with canine MRP1.
免疫原
non-denatured membrane preparations of H69AR human small cell lung cancer cell line, which highly expresses MRP1. The epitope resides within amino acids 918-924 of human MRP1.
应用
Monoclonal Anti-MRP1 antibody produced in mouse has been used in:
- western blotting
- immunohistochemistry
- flow cytometry
- immunoprecipitation
- immunocytochemistry
包装
200 μL in glass insert
生化/生理作用
Multidrug resistance associated protein 1 (MRP1) functions in the metabolism of many drugs. It is involved in transporting different biomolecules and anticancer drugs like prostaglandins, glutathione, alkaloids and anthracyclines. MRP1 offers resistance to chemotherapy to cells in solid tumors.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD01865488 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | QCRL-1, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~190 kDa |
| species reactivity | human |
| 浓度 | ~2 mg/mL |
| technique(s) | flow cytometry: suitable immunocytochemistry: 10-20 μg/mL using cultured HeLa cells or HepG2 human hepatocellular carcinoma cells immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P33527 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... ABCC1(4363) |
