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TAO2 Protein, active, 10 μg

品牌
Sigma-Aldrich
货号
14-736-M
规格纯度
Active, N-terminal 6His-tagged, recombinant, TAO2. TAO2 is a MAP3K that doubly phosphorylates & activates the MAP2Ks, MEK3 & MEK6. For use in Kinase Assays.
参考价格
51842.9 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Product Source: expressed in Sf21 cells
N-terminal 6His-tagged, recombinant, TAO2. TAO2 is a mitogen-activated protein kinase kinase kinase (MAP3K) that doubly phosphorylates and activates the MAP kinase kinases (MAP2Ks) MEK3 and MEK6.

应用

Research Category
Metabolism
Research Sub Category
Cardiovascular Disease

Metabolic Disorders

生化/生理作用

Protein Target: TAO2
Target Sub-Family: STE

质量

Routinely evaluated by phosphorylation of 0.8 mg/ml myelin basic protein (MBP). Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of TAO2.

单位定义

Specific Activity: where one unit of TOA2 activity is defined as 1 nmol phosphate incorporated into 0.8 mg/mL myelin basic per minute at 30°C with a final ATP concentration of 100 μM.

外形

10 μg of enzyme in 100 μL of 50 mM Tris/HCl pH7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Ni2+/NTA-agarose

储存及稳定性

Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160405
NACRESNA.32

产品性质

质量水平100
生物来源human
测定84% (Coomassie blue staining, SDS-PAGE)
specific activity1562 U/mg
分子量Mw 138.3 kDa
manufacturer/tradenameChemicon®
technique(s)activity assay: suitable (kinase)
NCBI登记号NM_004783.2
NM_016151.2
UniProt登记号Q9UL54
Gene Informationhuman ... TAOK2(9344)

安全信息

象形图GHS07
警示用语:Warning
危险声明H317
预防措施声明P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
危险分类Skin Sens. 1
储存分类代码10 - Combustible liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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