产品说明
一般描述
The SCF (Skp1-Cul1-F-box protein) complexes represent the largest family of ubiquitin-protein ligases and mediate the ubiquitination of a broad spectrum of regulatory and signalling proteins in diverse cellular pathways. The SCF consists of three invariant components, Skp1, Cul1, and Rbx1 and an interchangeable subunit, an F-box protein which is responsible for recruiting specific substrates to be ubiquitinated by the SCF. SCFFbw7 mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell division, growth and differentiation, including Cyclin E1, c-Myc, Notch and c-Jun. Mutations have been found in the Fbw7 subunit of SCFFbw7 in various types of human cancers, therefore Fbw7 is considered to be a potent tumour suppressor.
N-terminal c-Myc, 6His-tagged, recombinant human Smad7 full length, expressed by baculovirus in Sf21 insect cells.
应用
Ubiquitination Cascade Comoponent: E3
外形
Purified using glutathione sepharose.
其他说明
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160405 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | human |
| manufacturer/tradename | Upstate® |
| technique(s) | activity assay: suitable |
| NCBI登记号 | NM_003592 NM_014248 NM_033632 NM_170679 |
| Gene Information | human ... Cul1(8454), Fbw7(55294), Rbx1(9978), Skp1(6500) |
