产品说明
一般描述
N-Terminal GST-tagged, recombinant, human Ret amino acids 658 – end containing the V804L mutation.
The V804L mutation has been identified in patients with familial medullary thyroid carcinoma (FMTC) (Lombardo F. et al., (2002), JCEM, 87, 1674-1680; Lesueur F. et al, (2005), JCEM, 90, 3454-3457).
Product Source: Expressed by baculovirus in Sf21 insect cells.
应用
Research Category
Inflammation & Immunology
生化/生理作用
Target Sub-Family: TK
Protein Target: Ret
包装
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-758M when ordering the 250 µg size.
质量
Routinely evaluated by phosphorylation of CHKtide.
外形
Glutathione agarose affinity chromatography
50mM Tris-HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
储存及稳定性
Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
其他说明
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160405 |
| NACRES | NA.32 |
产品性质
| 质量水平 | 300 100 |
| 生物来源 | human |
| 分子量 | Mw 79.2 kDa |
| manufacturer/tradename | Upstate® |
| technique(s) | activity assay: suitable (kinase) |
| NCBI登记号 | NM_000323 |
| UniProt登记号 | P07949 P35546 (mouse) |
| Gene Information | human ... RET(5979) |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H317 |
| 预防措施声明 | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| 危险分类 | Skin Sens. 1 |
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
