产品说明
一般描述
Product Source: Recombinant enzyme expressed in Sf21 cells
N-terminal 6His-tagged, recombinant, human Haspin, amino acids 471-end, expressed by baculovirus in Sf21 insect cells.
应用
Research Category
Metabolism
Research Sub Category
Cardiovascular Disease
Metabolic Disorders
生化/生理作用
Protein Target: haspin
Target Sub-Family: Misc
质量
Routinely evaluated by phosphorylation of 500 μM RARTLSFAEPG.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of Haspin.
单位定义
Specific Activity: where one unit of Haspin activity is defined as 1 nmol phosphate incorporated into 500 μM RARTLSFAEPG per minute at 30°C with a final ATP concentration of 100 μM.
外形
10 μg of enzyme in 6.3 μL of 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Purified using Ni2+/NTA agarose. Purity 83% by SDS-PAGE and Coomassie blue staining.
储存及稳定性
Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160405 |
| NACRES | NA.32 |
产品性质
| 质量水平 | 300 100 |
| 生物来源 | human |
| specific activity | 2237 U/mg |
| 分子量 | Mw 41.1 kDa |
| manufacturer/tradename | Upstate® |
| technique(s) | activity assay: suitable (kinase) |
| NCBI登记号 | NM_031965.2 |
| UniProt登记号 | Q8TF76 |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H317 |
| 预防措施声明 | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| 危险分类 | Skin Sens. 1 |
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
