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Abl (Q252H) Protein, active, 10 μg

品牌
Sigma-Aldrich
货号
14-751-M
规格纯度
Active, N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the Q252H mutation. For use in Kinase Assays.
参考价格
7211.57 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the Q252H mutation. The Abl tyrosine kinase inhibitor STI571 is an effective therapy for stable-phase chronic myeloid leukemia patients. Many patients responding to STI571 later relapse due to a reactivation of Bcr-Abl activity. In certain cases this appears to correlate with the presence of the Q252H mutation, which confers resistance to drug binding (Shah et al., (2002), Cancer Cell 2, 117-125).
Product Source: Expressed by baculovirus in Sf21 insect cells.

应用

Research Category
Metabolism
Research Sub Category
Cardiovascular Disease

Metabolic Disorders

生化/生理作用

Protein Target: Abl
Target Sub-Family: TK

质量

Routinely evaluated by phosphorylation of 50 μM Abltide. Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of active Abl (Q252H).

单位定义

Specific Activity: one unit of Abl (Q252H) activity is defined as 1 nmol phosphate incorporated into 50 μM Abltide (EAIYAAPFAKKK) per minute at 30°C with a final ATP concentration of 100 μM.

外形

Ni2+/NTA-agarose
10 μg of enzyme in 100 μL of 50 mM Tris/HCl pH7.5, 150 mM NaCl, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1 mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij 35. Frozen solution.

储存及稳定性

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160405
NACRESNA.32

产品性质

质量水平300
100
生物来源human
测定51% (Coomassie staining, SDS-PAGE)
specific activity1089 U/mg
分子量Mw 121 kDa
manufacturer/tradenameUpstate®
technique(s)activity assay: suitable (kinase)
NCBI登记号NM_005157.3
NM_007313.2
UniProt登记号P00519

安全信息

象形图GHS07
警示用语:Warning
危险声明H317
预防措施声明P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
危险分类Skin Sens. 1
储存分类代码10 - Combustible liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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