产品说明
一般描述
A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein compound (DAF-2T, Cat. No. 251510). Can detect NO under neutral conditions (detection limit = 5 nM).
A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vivo. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent compound triazolofluorescein (DAF-2 T). Can detect NO under neutral conditions (detection limit = 5 nM). Excitation maximum: 495 nm; emission maximum: 515 nm.
生化/生理作用
Primary Target
Fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.
警告
Toxicity: Irritant (B)
外形
A 5 mM (1 mg in 550 µl) solution in DMSO.
重悬
Following initial thaw, aliquot and freeze (-20°C).
其他说明
Berkels, R., et al. 2000. Cell Calcium27, 281.
Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.
法律信息
Manufactured by Daiichi Pure Chemicals Co., Ltd
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
经验(实验)分子式 | C20H14N2O5 |
分子量 | 362.34 |
MDL编号 | MFCD05865100 |
产品性质
质量水平 | 100 |
测定 | ≥98% (HPLC) |
形式 | solution |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze protect from light |
颜色 | pale yellow |
荧光 | λex ~495 nm λem ~515 nm |
储存温度 | −20℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
闪点(F) | 188.6 °F - (Dimethylsulfoxide) |
闪点(C) | 87 ℃ - (Dimethylsulfoxide) |