产品说明
一般描述
Recombinant, Streptococcus pneumoniae β1,4-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,4-linked galactose. This enzyme is not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.
Note: 1 mU = 1 milliunit.
应用
Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-β-D-galactopyranoside per min at 37°C, pH 5.0.
外形
In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5. Sterile solution.
其他说明
Kierman, U.A., et al. 2004. Proteomincs4, 1825.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 酶学委员会(EC)编号 | 3.2.1.23 ( BRENDA | IUBMB ) |
产品性质
| 形式 | liquid |
| 质量水平 | 200 |
| specific activity | ≥1.5 units/mL ≥6 units/mg protein |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | do not freeze |
| 异质活性 | N-acetylglucosaminidase, α-galactosidase, α-mannosidase, neuraminidases, proteases, none detected |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
