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Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1)

品牌
Sigma-Aldrich
货号
OP43L
规格纯度
lyophilized, clone DO-1, Calbiochem®
参考价格
6432.04 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Recognizes the ~53 kDa wild-type and mutant p53 protein in A431 cells and breast carcinoma tissue.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with X63Ag8.653 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant and wild-type forms of p53.
This Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) is validated for use in Frozen sections, Gel Shift, Immunoblotting, ICC, IP, Paraffin sections for the detection of p53 (Ab-6) (Pantropic).

免疫原

wild type, recombinant, human p53
Epitope: Within amino acids 21-25 of human p53
Human

包装

100 μg in Plastic ampoule

应用

Frozen sections (1 µg/ml; see application references)

Gel Shift (see comments)

Immunoblotting (0.1 µg/ml; see application references)

Immunocytochemistry (1-2.5 µg/ml; see application references)

Immunoprecipitation (1 µg/ml or use Cat. No. OP43A; see application references)

Paraffin sections (1 µg/ml, pepsin, heat or pressure cooker pre-treatment required; see application references)

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from a volatile buffer, 100 µg BSA.

重悬

We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 μg/ml. Reconstituted product will be more stable if 0.1% sodium azide is added (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least two hours. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.

分析说明

Negative Control
SK-OV-3 cells or normal skin tissue
Positive Control
A431 cells or breast carcinoma tissue

其他说明

El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Legros, Y., et al. 1994. Oncogene9, 2071.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kastan, M.B., et al. 1992. Cell71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Lane, D.P. 1992. Nature358, 15.
Vojtesek, B., et al. 1992. J. Immunol. Meth.151, 237.
Kastan, M.B., et al. 1991 Cancer Res.51, 6304.
Recognizes both mutant and wild-type p53 under denaturing and non-denaturing conditions. This antibody reacts weakly with rodent p53; we do not recommend it for rodent samples. For gel shift assay, resuspend in 100 µl of buffer. Wild-type p53 has a short half life and is present in low amounts in cells. For immunoprecipitation increasing the amount of sample and labeling with 35S-Met for less than or equal to 1 h will aid in visualizing wild-type p53. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆DO-1, monoclonal
形式lyophilized
不包含 preservative
species reactivityfeline, human
should not react with rat, mouse
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
同位素/亚型IgG2a
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 3

Sigma-Aldrich

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