位置:首页 > 品牌 > Sigma-Aldrich >

Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421)

品牌
Sigma-Aldrich
货号
OP03L
规格纯度
lyophilized, clone PAb421, Calbiochem®
参考价格
6190.47 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Purified mouse monoclonal antibody, originally called clone L21, generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells (see application references). Recognizes the ~53 kDa wild-type and mutant forms of p53.
Recognizes the ~53 kDa mammalian wild-type and mutant p53 protein. Does not recognize phosphorylated p53.
This Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) is validated for use in Frozen Sections, Gel Shift, Immunoblotting, IF, FC, IP for the detection of p53 (Ab-1) (Pantropic).

免疫原

partially purified mouse p53
Mouse
Epitope: within amino acids 376-378 of human p53

包装

100 μg in Plastic ampoule

应用

Frozen Sections (10 µg/ml, see application references)

Gel Shift (see comments)

Immunoblotting (10 µg/ml, see application references)

Immunofluorescence (1-20 µg/ml, see application references)

Flow Cytometry (1-20 µg/ml)

Immunoprecipitation (1 µg per sample, see application references)

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from a volatile buffer, 100 µg BSA.

重悬

Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide; freezing of aliquots is best for long-term storage of reconstituted product, but repetitive freezing and thawing should be avoided.

分析说明

Negative Control
Normal skin tissue or SK-OV-3 cells
Positive Control
Breast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)

其他说明

Originally called clone L21. Does not recognize phosphorylated or acetylated p53. For a gel shift assay, resuspend in 100 µl buffer. Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53, and short incubation times with 35S-Met (≤1 h) will help reduce background. For immunoblotting wild-type p53, maximize sensitivity by concentrating samples by immunoprecipitation with Cat. No. OP03, then perform immunoblotting using Cat. No. PC35 and chemiluminescent detection. Antibody should be titrated for optimal results in individual systems.
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Legros, Y., et al. 1994. Oncogene9, 2071.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Kastan, M.B., et al. 1992. Cell71, 587.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51 6304.
Crawford, L. and Harlow, E. 1982. J. Virol.41, 709.
Harlow, E., et al. 1981. J. Virol.39 861.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆PAb421, monoclonal
形式lyophilized
不包含 preservative
species reactivitymonkey, rabbit, mouse, human, rat
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
同位素/亚型IgG2a
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 3

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号