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Anti-Cathepsin B (Ab-1) Mouse mAb (CA10)

品牌
Sigma-Aldrich
货号
IM27L
规格纯度
lyophilized, clone CA10, Calbiochem®
参考价格
6747.92 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/O-NS1 plasmacytoma cells. Recognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B.
Anti-Cathepsin B (Ab-1), mouse monoclonal, clone CA10, recognizes the ~43 kDa latent and ~31 kDa active forms of cathepsin B in HepG2 cells. It is validated for WB, IF, and IP.
Recognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B in HepG2 cells.

免疫原

purified human liver cathepsin B
Human
Epitope: within the heavy chain of mature cathepsin B

包装

100 μg in Glass bottle

应用

Immunoblotting (250 ng/ml)

Immunoprecipitation (1 µg/ml)

Immunofluorescence (see comments)

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from 20 mM ammonium bicabonate buffer, 100 µg BSA (100 µg size) or 20 µg BSA (20 µg size).

重悬

Resuspend the lyophilized antibody with 1 ml sterile PBS. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long-term storage of reconstituted product. Avoid freeze/thaw cycles.

分析说明

Positive Control
Hep G2 cells

其他说明

Sloane, B.F. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1:137-152.
Barret, A.J. 1987. Trends Biochem. Sci.12, 193.
Chan, S.J., et al. 1986. Proc. Natl. Acad. Sci. USA83, 7721.
Barrett, A.J. and Kirschke, H. 1981. Meth. Enzymol.80, 535.
This antibody recognizes an epitope on the heavy chain of mature cathepsin B and therefore detects both mature and pro-cathepsin B. Also reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆CA10, monoclonal
形式lyophilized
不包含 preservative
species reactivityhuman, bovine, porcine
should not react with rat
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
同位素/亚型IgG2a
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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