产品说明
生化/生理作用
PDK1 (3-phosphoinositide-dependent protein kinase) is activated by the presence of PtdIns (3, 4, 5) P3 or PtdIns (3, 4) P2. PDK1 then activates protein kinase B (PKB) which, in turn, inactivates glycogen synthase kinase-3 (GSK3). The phosphorylation of other proteins by PKB and GSK3 is likely to mediate many of the intracellular actions of insulin. Thus, PDK1 plays a key role in mediating many of the actions of the second messenger(s) PtdIns (3, 4, 5) P3 and/or PtdIns (3, 4) P2. The human PDK1 is a 556-residue monomeric enzyme comprising of a catalytic domain that is most similar to the PKA, PKB and PKC subfamily of protein kinases.
PDPK1 (3-phosphoinositide dependent protein kinase 1) is a serine/threonine protein kinase which is activated upon accumulation of PIP3 (phosphatidylinositol-3,4,5-trisphosphate), a PI3K (phosphoinositide 3-kinase) product. It controls AGC (protein kinase A, G, and C) kinase members, such as AKT (v-akt murine thymoma viral oncogene homolog), p70 ribosomal S6 kinase (S6K), serum- and glucocorticoid-induced protein kinase (SGK), and protein kinase C (PKC) members. It is involved in metabolism, growth, proliferation, and survival. PDPK1 is associated with growth of angiosarcoma cells.
外形
Supplied in 50mM NaPhosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1mM PMSF, 0.2 mM DTT, 25% glycerol.
法律信息
PRECISIO is a registered trademark of sigma-aldrich Co. LLC
基本信息
| NACRES | NA.32 |
产品性质
| 质量水平 | 200 |
| 重组 | expressed in baculovirus infected Sf9 cells |
| 产品线 | PRECISIO® Kinase |
| 测定 | ≥70% (SDS-PAGE) |
| 形式 | buffered aqueous glycerol solution |
| specific activity | 102-138 nmol/min·mg |
| 分子量 | ~67 kDa |
| UniProt登记号 | Q6A1A2 O15530 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... PDPK1(5170) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
