产品说明
一般描述
Isocitrate dehydrogenase 1 (IDH1) gene is located on human chromosome 2q33. IDH1 is present in the cytoplasm and peroxisomes. It is expressed at high levels in the mammalian liver. IDH1 consists of a C-terminal tripeptide peroxisome, that targets the signal one sequence.
生化/生理作用
Isocitrate dehydrogenase 1 (IDH1) help in the oxidative carboxylation of isocitrate to α-ketoglutarate, that results in the formation of reduced nicotinamide adenine dinucleotide phosphate (NADPH). It participates in lipid metabolism and glucose sensing. Suppressing IDH1 in pancreatic islets affects the insulin secretion, induced by glucose and leads to increase in lactate production.
单位定义
One unit corresponds to the amount of enzyme which converts 1.0 μmol DL-isocitrate to α-ketoglutarate per minute at pH 7.4 and 37 ℃ (NADP as cofactor).
外形
Supplied as a lyophilized powder containing Tris-HCl pH 8.0, trehalose, ammonium sulfate and DTT
基本信息
| 酶学委员会(EC)编号 | 1.1.1.42 ( BRENDA | IUBMB ) |
| NACRES | NA.54 |
产品性质
| 质量水平 | 200 |
| 重组 | expressed in E. coli |
| 测定 | ≥90% (SDS-PAGE) |
| 形式 | lyophilized powder |
| specific activity | ≥80 units/mg protein |
| 分子量 | 46,658 Da |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
