产品说明
生化/生理作用
Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β2), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.
外形
Supplied as a 0.2 μm filtered solution in 50 mM sodium acetate, pH 4, with BSA as a carrier protein.
制备说明
Recombinant, rat Interleukin-6 is produced from a DNA sequence encoding the mature rat IL-6 protein. The methionyl form of mature rat IL-6, a 188 amino acid protein, has a predicted molecular mass of approx. 22 kDa. Rat and mouse IL-6 share 93% amino acid sequence identity.
分析说明
The biological activity is measured in a cell proliferation assay using IL-6 dependent mouse plasmacytoma cell line, T1165.85.2.1.
基本信息
| MDL编号 | MFCD01323739 |
产品性质
| 质量水平 | 200 |
| 生物来源 | rat |
| 重组 | expressed in E. coli |
| 测定 | >97% (SDS-PAGE) |
| 形式 | buffered aqueous solution |
| 效能 | 0.01-0.06 ng/mL ED50 |
| 质量 | endotoxin tested |
| 分子量 | predicted mol wt ~22 kDa |
| 包装 | pkg of 10 and 50 μg |
| technique(s) | cell culture | mammalian: suitable |
| 杂质 | <0.01EU/mg (LAL test) |
| UniProt登记号 | P20607.1 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | rat ... Il6(24498) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
